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Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

7,916 Views

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11:35 min

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May 04, 2018

DOI:

10.3791/57298-v

DOI:
10.3791/57298-v

•

11:35 min
May 04, 2018

•

2 Views
, , , , , , ,
1Department of Pediatrics, Mucosal Immunology and Biology Research Center,Massachusetts General Hospital, 2Department of Molecular Biology,Massachusetts General Hospital, 3Department of Genetics,Harvard Medical School, 4Department of Pediatrics,Harvard Medical School

Summary

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Pseudomonas aeruginosa infection causes significant morbidity in vulnerable hosts. The nonredundant transposon insertion mutant library of P. aeruginosa strain PA14, designated as PA14NR Set, facilitates analysis of gene functionality in numerous processes. Presented here is a protocol to generate high-quality copies of the PA14NR Set mutant library.

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Drenkard, E., Hibbler, R. M., Gutu, D. A., Eaton, A. D., Silverio, A. L., Ausubel, F. M., Hurley, B. P., Yonker, L. M. Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants. J. Vis. Exp. (135), e57298, doi:10.3791/57298 (2018).

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