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Isolamento e caratterizzazione di cellule Stromal Mesenchymal cardiache da Endomyocardial campioni bioptici di pazienti cardiomiopatia aritmogena
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
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Isolamento e caratterizzazione di cellule Stromal Mesenchymal cardiache da Endomyocardial campioni bioptici di pazienti cardiomiopatia aritmogena

italiano

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7,549 Views

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09:16 min

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February 28, 2018

DOI:

10.3791/57263-v

DOI:
10.3791/57263-v

•

09:16 min
February 28, 2018

•

14 Views
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1Vascular Biology and Regenerative Medicine Unit,Centro Cardiologico Monzino-IRCCS, 2Department of Clinical Sciences and Community Health,Università degli Studi di Milano, 3Heart Rhythm Center,Centro Cardiologico Monzino-IRCCS

Summary

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In questo articolo viene fornito un metodo per isolare cellule stromale mesenchymal cardiache da endomyocardial campioni bioptici di pazienti cardiomiopatia aritmogena. Loro caratterizzazione e il protocollo per aumentare la loro differenziazione adipogenico sono descritti.

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Cite this Article

Pilato, C. A., Stadiotti, I., Maione, A. S., Saverio, V., Catto, V., Tundo, F., Dello Russo, A., Tondo, C., Pompilio, G., Casella, M., Sommariva, E. Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients. J. Vis. Exp. (132), e57263, doi:10.3791/57263 (2018).

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