Journal
/
발생학
/
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
JoVE 신문
발생학
This content is Free Access.
JoVE 신문 발생학
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

7,549 Views

•

09:16 min

•

February 28, 2018

DOI:

10.3791/57263-v

DOI:
10.3791/57263-v

•

09:16 min
February 28, 2018

•

14 Views
, , , , , , , , , ,
1Vascular Biology and Regenerative Medicine Unit,Centro Cardiologico Monzino-IRCCS, 2Department of Clinical Sciences and Community Health,Università degli Studi di Milano, 3Heart Rhythm Center,Centro Cardiologico Monzino-IRCCS

Summary

Automatically generated

In this article, a method to isolate cardiac mesenchymal stromal cells from endomyocardial bioptic samples of arrhythmogenic cardiomyopathy patients is provided. Their characterization and the protocol to boost their adipogenic differentiation are described.

Explore More Videos

Cardiac Mesenchymal Stromal CellsArrhythmogenic CardiomyopathyEndomyocardial BiopsyAdipogenic DifferentiationPhenotypingCell IsolationCell CultureCollagenase DigestionSurface Markers

Read Article

Cite this Article

Pilato, C. A., Stadiotti, I., Maione, A. S., Saverio, V., Catto, V., Tundo, F., Dello Russo, A., Tondo, C., Pompilio, G., Casella, M., Sommariva, E. Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients. J. Vis. Exp. (132), e57263, doi:10.3791/57263 (2018).

Download .ris file

Copy

Share Video

.

Copy