Journal
/
发育生物学
/
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

DOI:
10.3791/57263-v

•

09:16 min

•

February 28, 2018

•

, , , , , , , , , ,
1Vascular Biology and Regenerative Medicine Unit,Centro Cardiologico Monzino-IRCCS, 2Department of Clinical Sciences and Community Health,Università degli Studi di Milano, 3Heart Rhythm Center,Centro Cardiologico Monzino-IRCCS

Chapters

  • 00:04Title
  • 01:12Cardiac Biopsy Collection and Processing
  • 03:06Characterizing Cardiac Mesenchymal Stromal Cells (C-MSC) by Flow Cytometry
  • 05:22Oil Red O (ORO) Staining C-MSC after Adipogenic Differentiation
  • 07:02Results: Flow Cytometry Analysis and ORO Staining
  • 08:27Conclusion

Summary

自动翻译

自动翻译

In this article, a method to isolate cardiac mesenchymal stromal cells from endomyocardial bioptic samples of arrhythmogenic cardiomyopathy patients is provided. Their characterization and the protocol to boost their adipogenic differentiation are described.

Tags

Keywords: Cardiac Mesenchymal Stromal CellsArrhythmogenic CardiomyopathyEndomyocardial BiopsyAdipogenic DifferentiationPhenotypingCell IsolationCell CultureCollagenase DigestionSurface Markers

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/57263?article_type=v

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells
check_url/cn/57263?article_type=v

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
check_url/cn/57263?article_type=v

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
check_url/cn/57263?article_type=v

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
check_url/cn/57263?article_type=v

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
check_url/cn/57263?article_type=v

Isolation and Characterization of Single Cells from Zebrafish Embryos
check_url/cn/57263?article_type=v

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
check_url/cn/57263?article_type=v

Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
check_url/cn/57263?article_type=v

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
check_url/cn/57263?article_type=v

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

Read Article