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使用斑马鱼作为体内模型系统分析 DNA 复制定时

JoVE Journal
Genetics

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JoVE Journal Genetics

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

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使用斑马鱼作为体内模型系统分析 DNA 复制定时

DOI:

10.3791/57146-v

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10:17 min

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April 30, 2018

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Joseph C. Siefert², Emily A. Clowdus², Duane Goins, Amnon Koren, Christopher L. Sansam²

¹Cell Cycle and Cancer Biology Research Program,Oklahoma Medical Research Foundation, ²Department of Cell Biology,University of Oklahoma Health Sciences Center, ³Department of Molecular Biology and Genetics,Cornell University

Chapters

00:05Title
01:01Setting Up Adult Zebrafish for Breeding
02:01Timed Matings – Collecting, Sorting, and Housing Zebrafish Embryos for Experiments
03:23Dechorionate, Deyolk, and Fix Zebrafish Embryos
06:09Staining DNA and FACS Sorting Embryos
08:00Results: Genome-wide DNA Replication Timing at High-resolution in Zebrafish
09:28Conclusion

Summary

Automatic Translation

斑马鱼最近被用作一个体内模型系统, 用于研究开发过程中 DNA 复制的时机。这里详细介绍了使用斑马鱼胚胎来描述复制时间的协议。该协议可以很容易地适应于研究突变体、个体细胞类型、疾病模型和其他物种的复制时间。

Tags

DNA Replication Timing Zebrafish In Vivo Model System Cell Cycle Chromatin Biology Embryonic Development Single-cell Suspensions Breeding Strategies Timed Matings Embryo Collection Synchronous Development

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