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Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
JoVE Journal
Genetics
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JoVE Journal Genetics
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

English

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5,531 Views

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10:56 min

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January 16, 2018

DOI:

10.3791/56898-v

DOI:
10.3791/56898-v

•

10:56 min
January 16, 2018

•

1 Views
, , , , , , ,
1Laboratory of tRNA Biology, Department of Biology,College of Charleston, 2Department of Biochemistry and Molecular Biology,MUSC, 3Department of Biology,College of Charleston

Summary

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We describe an original transfer RNA analysis platform named SPOt (Streamlined Platform for Observing tRNA). SPOt simultaneously measures cellular levels of all tRNAs in biological samples, in only three steps, and in less than 24 hours.

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Cite this Article

Emetu, S., Troiano, M., Goldmintz, J., Tomberlin, J., Grelet, S., Howe, P. H., Korey, C., Geslain, R. Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays. J. Vis. Exp. (131), e56898, doi:10.3791/56898 (2018).

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