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Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
JoVE Journal
Genetics
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JoVE Journal Genetics
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

DOI:
10.3791/56898-v

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10:56 min

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January 16, 2018

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, , , , , , ,
1Laboratory of tRNA Biology, Department of Biology,College of Charleston, 2Department of Biochemistry and Molecular Biology,MUSC, 3Department of Biology,College of Charleston

Chapters

  • 00:05Title
  • 01:06Culture and Metabolic Labeling
  • 02:23Preparation of Total RNAs
  • 04:08Preparation of 96-well Printer Plates
  • 04:38Array Printing
  • 07:26Array Hybridization and Quantification
  • 10:02Conclusion

Summary

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We describe an original transfer RNA analysis platform named SPOt (Streamlined Platform for Observing tRNA). SPOt simultaneously measures cellular levels of all tRNAs in biological samples, in only three steps, and in less than 24 hours.

Tags

Keywords: Metabolic LabelingTRNA ProfilingMacroarray AnalysisTRNA RegulationProtein SynthesisMycobacterium SmegmatisPhosphorus-32 LabelingRNA ExtractionChloroform Extraction

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