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Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
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Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

7,691 Views

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10:17 min

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April 30, 2018

DOI:

10.3791/57146-v

DOI:
10.3791/57146-v

•

10:17 min
April 30, 2018

•

8 Views
, , , ,
1Cell Cycle and Cancer Biology Research Program,Oklahoma Medical Research Foundation, 2Department of Cell Biology,University of Oklahoma Health Sciences Center, 3Department of Molecular Biology and Genetics,Cornell University

Summary

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Zebrafish were recently used as an in vivo model system to study DNA replication timing during development. Here is detailed the protocols for using zebrafish embryos to profile replication timing. This protocol can be easily adapted to study replication timing in mutants, individual cell types, disease models, and other species.

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DNA Replication TimingZebrafishIn Vivo Model SystemCell CycleChromatin BiologyEmbryonic DevelopmentSingle-cell SuspensionsBreeding StrategiesTimed MatingsEmbryo CollectionSynchronous Development

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Cite this Article

Siefert, J. C., Clowdus, E. A., Goins, D., Koren, A., Sansam, C. L. Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System. J. Vis. Exp. (134), e57146, doi:10.3791/57146 (2018).

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