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人雌性生殖道树突状细胞表型鉴定的分离及功能研究
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies
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人雌性生殖道树突状细胞表型鉴定的分离及功能研究

DOI:
10.3791/57100-v

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12:32 min

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March 13, 2018

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, , ,
1Department of Microbiology and Immunology,Geisel School of Medicine at Dartmouth

Chapters

  • 00:04Title
  • 01:07Enzymatic Digestion of Tissues
  • 02:34Physical Separation of Single Cells
  • 04:57Dead Cell Removal
  • 06:00DC Purification by Positive Magnetic Bead Selection
  • 08:02Allogeneic Stimulation Assay to Assess DC Cell Function
  • 10:19Results: Successful Isolation of DCs from the Human Female Reproductive Tract
  • 11:55Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了一种从人类女性生殖道的不同解剖间隔分离纯化树突状细胞的方法, 以评价其表型鉴定和功能特征。该方法可用于隔离其他粘膜组织中的其他免疫细胞或树突状细胞。

Tags

Dendritic CellsFemale Reproductive TractPhenotypicalFunctionalImmune CellsEpithelial SheetsEnzymatic DigestionCell Isolation

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