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Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

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22,410 Views

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06:09 min

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June 22, 2016

DOI:

10.3791/54128-v

DOI:
10.3791/54128-v

•

06:09 min
June 22, 2016

•

16 Views
, , ,
1Translational Immunology, Institute of Molecular and Cell Biology,Agency for Science, Technology and Research, 2Singapore Immunology Network, 3Institute of Biomedical Studies,Baylor University

Summary

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Immature dendritic cells can be selectively differentiated into tolerogenic or mature dendritic cells to regulate the balance between immunity and tolerance. This work presents a means to generate from immature monocyte derived dendritic cells (moDCs), in vitro tolerogenic and mature moDCs that differ in metabolic phenotypes.

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Cite this Article

Sim, W. J., Malinarich, F., Fairhurst, A., Connolly, J. E. Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes. J. Vis. Exp. (112), e54128, doi:10.3791/54128 (2016).

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