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Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies
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Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies
Chapters
- 00:04Title
- 01:07Enzymatic Digestion of Tissues
- 02:34Physical Separation of Single Cells
- 04:57Dead Cell Removal
- 06:00DC Purification by Positive Magnetic Bead Selection
- 08:02Allogeneic Stimulation Assay to Assess DC Cell Function
- 10:19Results: Successful Isolation of DCs from the Human Female Reproductive Tract
- 11:55Conclusion
We describe here a method to isolate and purify dendritic cells from different anatomical compartments in the human female reproductive tract for the evaluation of their phenotypical and functional characteristics. This method can be adapted to isolate other immune cells or dendritic cells from other mucosal tissues.
