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Un protocolo fácil de generar proteínas Site-Specifically acetiladas en Escherichia Coli
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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Un protocolo fácil de generar proteínas Site-Specifically acetiladas en Escherichia Coli

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6,749 Views

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11:08 min

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December 09, 2017

DOI:

10.3791/57061-v

DOI:
10.3791/57061-v

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11:08 min
December 09, 2017

•

1 Views
, , , ,
1Department of Chemistry and Biochemistry,University of Arkansas, 2Cell and Molecular Biology Program,University of Arkansas, 3Department of Biological Sciences,University of Arkansas, 4Fayetteville High School

Summary

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Expansión del código genético sirve como una poderosa herramienta para el estudio de una amplia gama de procesos biológicos, incluyendo acetilación de proteínas. Aquí demostramos un protocolo fácil aprovechar esta técnica para generar homogéneo acetilado proteínas en sitios específicos en células de Escherichia coli .

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Venkat, S., Gregory, C., Meng, K., Gan, Q., Fan, C. A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli. J. Vis. Exp. (130), e57061, doi:10.3791/57061 (2017).

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