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A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

English

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6,749 Views

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11:08 min

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December 09, 2017

DOI:

10.3791/57061-v

DOI:
10.3791/57061-v

•

11:08 min
December 09, 2017

•

1 Views
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1Department of Chemistry and Biochemistry,University of Arkansas, 2Cell and Molecular Biology Program,University of Arkansas, 3Department of Biological Sciences,University of Arkansas, 4Fayetteville High School

Summary

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Genetic code expansion serves as a powerful tool to study a wide range of biological processes, including protein acetylation. Here we demonstrate a facile protocol to exploit this technique for generating homogeneously acetylated proteins at specific sites in Escherichia coli cells.

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Cite this Article

Venkat, S., Gregory, C., Meng, K., Gan, Q., Fan, C. A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli. J. Vis. Exp. (130), e57061, doi:10.3791/57061 (2017).

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