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Un protocollo Facile generare proteine site-specifically acetilati in Escherichia Coli
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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Un protocollo Facile generare proteine site-specifically acetilati in Escherichia Coli

italiano

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6,749 Views

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11:08 min

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December 09, 2017

DOI:

10.3791/57061-v

DOI:
10.3791/57061-v

•

11:08 min
December 09, 2017

•

1 Views
, , , ,
1Department of Chemistry and Biochemistry,University of Arkansas, 2Cell and Molecular Biology Program,University of Arkansas, 3Department of Biological Sciences,University of Arkansas, 4Fayetteville High School

Summary

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Codice genetico espansione serve come un potente strumento per studiare una vasta gamma di processi biologici, compreso l'acetilazione della proteina. Qui dimostriamo che un protocollo facile sfruttare questa tecnica per la generazione in modo omogeneo acetilato proteine in siti specifici in cellule di Escherichia coli .

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Cite this Article

Venkat, S., Gregory, C., Meng, K., Gan, Q., Fan, C. A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli. J. Vis. Exp. (130), e57061, doi:10.3791/57061 (2017).

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