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Ex Vivo Infecção da Mucosa Genital humano tecido linfoide e fêmea com vírus de imunodeficiência humana 1 e Histoculture
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Immunology and Infection
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JoVE Journal Immunology and Infection
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

Ex Vivo Infecção da Mucosa Genital humano tecido linfoide e fêmea com vírus de imunodeficiência humana 1 e Histoculture

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8,907 Views

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11:14 min

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October 12, 2018

DOI:

10.3791/57013-v

DOI:
10.3791/57013-v

•

11:14 min
October 12, 2018

•

2 Views
, , , ,
1Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital,Karolinska Institutet, 2Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development,National Institutes of Health

Summary

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Infecção de tecidos humanos com vírus de imunodeficiência humana (HIV) ex vivo fornece um valioso modelo 3D da patogênese do vírus. Aqui, descrevemos um protocolo para processar e infectar amostras de tecido da amígdalas humanas e mucosa genital feminina com HIV-1 e mantê-las em cultura em uma interface líquido-ar.

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Cite this Article

Introini, A., Vanpouille, C., Fitzgerald, W., Broliden, K., Margolis, L. Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture. J. Vis. Exp. (140), e57013, doi:10.3791/57013 (2018).

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