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Infecção por vírus Zika do cérebro Fetal humano culta células-tronco neurais para análise de Immunocytochemical
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Developmental Biology
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JoVE Journal Developmental Biology
Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

Infecção por vírus Zika do cérebro Fetal humano culta células-tronco neurais para análise de Immunocytochemical

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6,357 Views

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10:27 min

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February 05, 2018

DOI:

10.3791/56917-v

DOI:
10.3791/56917-v

•

10:27 min
February 05, 2018

•

1 Views
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1Department of Neuroscience and Cell Biology,University of Texas Medical Branch, 2Department of Neurology,Johns Hopkins University, 3Beijing Institute for Brain Disorders,Capital Medical University

Summary

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Este artigo detalha os métodos que são usados para expandir as células-tronco neurais cérebro fetal humano na cultura, bem como diferenciá-los em vários subtipos neuronais e astrócitos, com ênfase na utilização de células-tronco neurais para estudar a infecção pelo vírus Zika.

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Cite this Article

McGrath, E. L., Gao, J., Wu, P. Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis. J. Vis. Exp. (132), e56917, doi:10.3791/56917 (2018).

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