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Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

6,357 Views

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10:27 min

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February 05, 2018

DOI:

10.3791/56917-v

DOI:
10.3791/56917-v

•

10:27 min
February 05, 2018

•

1 Views
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1Department of Neuroscience and Cell Biology,University of Texas Medical Branch, 2Department of Neurology,Johns Hopkins University, 3Beijing Institute for Brain Disorders,Capital Medical University

Summary

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This article details the methods that are used to expand human fetal brain neural stem cells in culture, as well as how to differentiate them into various neuronal subtypes and astrocytes, with an emphasis on the use of neural stem cells to study Zika virus infection.

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Zika VirusHuman Fetal Brain Neural Stem CellsImmunocytochemical AnalysisIn VitroNeural Stem Cell DifferentiationInnate Immune ResponseTranslational ApplicationNeurological DeficitsCell CultureCell PassageTrypsinDNaseCentrifugation

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Cite this Article

McGrath, E. L., Gao, J., Wu, P. Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis. J. Vis. Exp. (132), e56917, doi:10.3791/56917 (2018).

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