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Medição de deformabilidade e heterogeneidade de glóbulos vermelhos no sangue por Ektacytometry
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1Department of Pediatrics,Saint Louis University School of Medicine, 2Molecular and Clinical Nutrition Section, Digestive Diseases Branch,National Institute of Diabetes and Digestive and Kidney Diseases, 3Molecular and Clinical Hematology Branch,National Institute of Diabetes and Digestive and Kidney Diseases, 4Sickle Cell Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 5Edward A. Doisy Department of Biochemistry and Molecular Biology,Saint Louis University School of Medicine, 6Red Cell Physiology Laboratory,New York Blood Center
Chapters
- 00:05Title
- 01:05Ektacytometer Set-up
- 01:48Measuring Deformability as a Function of Increasing Shear Stress
- 04:10Measuring Cellular Heterogeneity
- 05:20Osmotic Gradient Ektacytometry
- 07:16Results: Diffraction Pattern Distortion and Relationship to Hemoglobin
- 08:41Conclusion
Aqui nós apresentamos técnicas para medir a deformabilidade eritrócitos e heterogeneidade celular por ektacytometry. Estas técnicas são aplicáveis às investigações gerais de deformabilidade eritrócitos e investigações específicas de doenças do sangue caracterizadas pela presença de eritrócitos rígidas e deformáveis em circulação, tais como anemia falciforme.
