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Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila
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Behavior
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JoVE Journal Behavior
Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

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8,135 Views

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07:24 min

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April 24, 2018

DOI:

10.3791/56900-v

DOI:
10.3791/56900-v

•

07:24 min
April 24, 2018

•

6 Views
, ,
1School of Life Science,University of Science and Technology of China, 2State key Laboratory of Brain and Cognitive Science, Institute of Biophysics,Chinese Academy of Sciences, 3University of Chinese Academy of Sciences

Summary

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Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

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Cite this Article

Jiang, L., Zhan, Y., Zhu, Y. Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila. J. Vis. Exp. (134), e56900, doi:10.3791/56900 (2018).

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