Journal
/
Comportamento
/
Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila
JoVE Journal
Comportamento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Comportamento
Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

8,135 Views

•

07:24 min

•

April 24, 2018

DOI:

10.3791/56900-v

DOI:
10.3791/56900-v

•

07:24 min
April 24, 2018

•

6 Views
, ,
1School of Life Science,University of Science and Technology of China, 2State key Laboratory of Brain and Cognitive Science, Institute of Biophysics,Chinese Academy of Sciences, 3University of Chinese Academy of Sciences

Summary

Automatically generated

Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

Explore More Videos

Food IntakeDrosophilaQuantitative AssayThermogenetic ActivationOptogenetic ActivationHungerSatietyAppetiteAgarose PadSucroseBlue DyeFeeding ChamberTemperature

Read Article

Cite this Article

Jiang, L., Zhan, Y., Zhu, Y. Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila. J. Vis. Exp. (134), e56900, doi:10.3791/56900 (2018).

Download .ris file

Copy

Share Video

.

Copy