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Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila
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Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

DOI:
10.3791/56900-v

•

07:24 min

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April 24, 2018

•

, ,
1School of Life Science,University of Science and Technology of China, 2State key Laboratory of Brain and Cognitive Science, Institute of Biophysics,Chinese Academy of Sciences, 3University of Chinese Academy of Sciences

章

  • 00:05標題
  • 00:51Preparing the Feeding Chamber
  • 02:58Thermogenetic Activation
  • 03:44Optogenetic Activation
  • 04:48Colorimetric Quantification of Food Consumption
  • 06:08Results: Activated Taotie-GAL4 Neurons Increase Food Intake
  • 06:55Conclusion

概要

自動翻訳

自動翻訳

Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

タグ

Food IntakeDrosophilaQuantitative AssayThermogenetic ActivationOptogenetic ActivationHungerSatietyAppetiteAgarose PadSucroseBlue DyeFeeding ChamberTemperature

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