Journal
/
Bioquímica
/
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

9,852 Views

•

11:22 min

•

January 30, 2018

DOI:

10.3791/56896-v

DOI:
10.3791/56896-v

•

11:22 min
January 30, 2018

•

5 Views
, , ,
1Institute of Physical Chemistry,University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology

Summary

Automatically generated

Here, we present a protocol to obtain three-color smFRET data and its analysis with a 3D ensemble Hidden Markov Model. With this approach, scientists can extract kinetic information from complex protein systems, including cooperativity or correlated interactions.

Explore More Videos

Three-color Single-molecule FRETProtein InteractionsCooperativityQuantitative BiologyProtein ComplexesDynamic InteractionsFlow Chamber AssemblyPEG-biotin Functionalized SlideMulti-color Prism TIRF MicroscopeEMCCD Camera Configuration

Read Article

Cite this Article

Götz, M., Wortmann, P., Schmid, S., Hugel, T. Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions. J. Vis. Exp. (131), e56896, doi:10.3791/56896 (2018).

Download .ris file

Copy

Share Video

.

Copy