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对雄性欧洲鳗鲡 (安圭拉) 进行激素成熟和精子冷冻保存的处理和处理
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Developmental Biology
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JoVE Journal Developmental Biology
Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation
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对雄性欧洲鳗鲡 (安圭拉) 进行激素成熟和精子冷冻保存的处理和处理

DOI:
10.3791/56835-v

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08:24 min

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January 28, 2018

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, , , , , , , ,
1Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal,Universitat Politècnica de València, 2Department of Aquaculture, Institute of Aquaculture and Environmental Safety,Szent Istvan University

Chapters

  • 00:05Title
  • 00:11Introduction
  • 00:49Fish Maintenance and Hormonal Maturation
  • 01:59Samplings
  • 03:05Sperm Quality Evaluation
  • 04:21Sperm Freezing Solutions and Materials
  • 04:42Sperm Freezing Method
  • 05:26Thawing Method
  • 06:18Results
  • 07:09Conclusion

Summary

Automatic Translation

Automatic Translation

欧洲鳗鲡成熟和精子冷冻保存的协议在过去的几年里得到了改善。这篇文章描述了最佳的协议可利用人绒毛膜促性腺激素 (hCG) 诱导成熟和甲醇为剂。

Tags

European EelAnguilla AnguillaHormonal MaturationSperm CryopreservationHCG InjectionsTanaka’s ExtenderMethanol CryoprotectantSperm Motility

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