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发育生物学
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Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

DOI:
10.3791/56835-v

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08:24 min

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January 28, 2018

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, , , , , , , ,
1Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal,Universitat Politècnica de València, 2Department of Aquaculture, Institute of Aquaculture and Environmental Safety,Szent Istvan University

Chapters

  • 00:05Title
  • 00:11Introduction
  • 00:49Fish Maintenance and Hormonal Maturation
  • 01:59Samplings
  • 03:05Sperm Quality Evaluation
  • 04:21Sperm Freezing Solutions and Materials
  • 04:42Sperm Freezing Method
  • 05:26Thawing Method
  • 06:18Results
  • 07:09Conclusion

Summary

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Protocols for European eel maturation and sperm cryopreservation have been improved over the last years. This article describes the best protocol available using human chorionic gonadotropin (hCG) for inducing maturation and methanol as cryoprotectant.

Tags

European EelAnguilla AnguillaHormonal MaturationSperm CryopreservationHCG InjectionsTanaka’s ExtenderMethanol CryoprotectantSperm Motility

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