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Chapters
- 00:05Title
- 00:41Egg Laying and Female Larvae Selection
- 02:12Pupae Antibody Staining Preparation
- 04:24Immunohistochemistry
- 05:52Dissecting and Mounting Pupal Ovaries
- 06:49Results: Clear Antibody Staining that Reveals Drosophila Structure and Cellular Organization
- 07:12Conclusion
The Drosophila ovary is an excellent model system for studying stem cell niche development. Though methods for dissecting larval and adult ovaries have been published, pupal ovary dissections require different techniques that have not been published in detail. Here we outline a protocol for dissecting, staining, and mounting pupal ovaries.
