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Dissection and Staining of Drosophila Pupal Ovaries
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Dissection and Staining of Drosophila Pupal Ovaries

Dissection and Staining of Drosophila Pupal Ovaries

9,117 Views

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07:35 min

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March 02, 2018

DOI:

10.3791/56779-v

DOI:
10.3791/56779-v

•

07:35 min
March 02, 2018

•

10 Views
, ,
1Department of Biological Sciences,Columbia University, 2Department of Cell and Systems Biology,University of Toronto

Summary

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The Drosophila ovary is an excellent model system for studying stem cell niche development. Though methods for dissecting larval and adult ovaries have been published, pupal ovary dissections require different techniques that have not been published in detail. Here we outline a protocol for dissecting, staining, and mounting pupal ovaries.

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Cite this Article

Park, K. S., Godt, D., Kalderon, D. Dissection and Staining of Drosophila Pupal Ovaries. J. Vis. Exp. (133), e56779, doi:10.3791/56779 (2018).

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