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昆虫細胞 (sf9) 蛋白質機能の試金のための外国遺伝子の一過性発現
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Genetics
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JoVE Journal Genetics
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
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昆虫細胞 (sf9) 蛋白質機能の試金のための外国遺伝子の一過性発現

日本語

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12,027 Views

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11:12 min

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February 22, 2018

DOI:

10.3791/56693-v

DOI:
10.3791/56693-v

•

11:12 min
February 22, 2018

•

12 Views
, , , ,
1Department of Biotechnology and Animal Science,National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences,Chonbuk National University, 3Department of Entomology,National Taiwan University

Summary

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このプロトコルを記述する外国蛋白質を表現するまたは潜在的な外国蛋白質とその切り捨てられたのアミノ酸の抗アポトーシス活性を評価に使用することができます熱衝撃によるタンパク質発現システム (pDHsp/V5-彼/sf9 細胞システム)昆虫細胞での酸。

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Cite this Article

Chang, J., Lee, S. J., Kim, J. S., Wang, C., Nai, Y. Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay. J. Vis. Exp. (132), e56693, doi:10.3791/56693 (2018).

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