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June 12, 2019
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In this method, we quantify the binding affinity of RNA binding proteins (RBPs) to cognate and non-cognate binding sites using a simple, live, reporter assay in bacterial cells. The assay is based on repression of a reporter gene.
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Katz, N., Cohen, R., Atar, O., Goldberg, S., Amit, R. An Assay for Quantifying Protein-RNA Binding in Bacteria. J. Vis. Exp. (148), e59611, doi:10.3791/59611 (2019).
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