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Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
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Genetics
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JoVE Journal Genetics
Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions

Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions

English

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7,474 Views

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07:20 min

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August 31, 2018

DOI:

10.3791/58044-v

DOI:
10.3791/58044-v

•

07:20 min
August 31, 2018

•

1 Views
, ,
1Biological Sciences Department,Vanderbilt University

Summary

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The aphid Aphis nerii colonizes on highly-defended plants in the dogbane family (Apocyanaceae) and provides numerous opportunities to study plant-insect interactions. Here, we present a series of protocols for the maintenance of plant and aphid cultures, and the generation and analysis of molecular and -omic data for A. nerii.

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Birnbaum, S. S., Rinker, D. C., Abbot, P. Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions. J. Vis. Exp. (138), e58044, doi:10.3791/58044 (2018).

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