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粘附细胞培养的时间推移荧光显微镜对蛋白质降解率的单细胞定量研究
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Developmental Biology
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JoVE Journal Developmental Biology
Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
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粘附细胞培养的时间推移荧光显微镜对蛋白质降解率的单细胞定量研究

DOI:
10.3791/56604-v

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12:06 min

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February 04, 2018

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,
1UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences,École Polytechnique Fédérale de Lausanne (EPFL)

Chapters

  • 00:00Title
  • 01:42Cell seeding and pulse labeling of the SNAP-tag
  • 04:15Time-lapse microscopy
  • 05:23Image processing and analysis
  • 07:55Results
  • 10:13Conclusions

Summary

Automatic Translation

Automatic Translation

该协议描述了一种方法, 以确定蛋白质半生命的单一生活粘附细胞, 使用脉冲标记和荧光时间推移成像的捕捉标签融合蛋白。

Tags

Protein DegradationSingle-cellTime-lapse Fluorescence MicroscopyAdherent Cell CultureSNAP-tagProtein Half-lifeFluorescence ImagingProtein TurnoverHeterogeneityEndogenous Protein TaggingOverexpressionDNA Repair EnzymeBenzylguanineFluorescent DyeExponential DecayEmbryonic Stem CellsMonolayerCell-cell InteractionsN-cadherin

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