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February 04, 2018
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このプロトコルでは、蛋白質半減単一接着細胞、パルスのラベリングとスナップ タグ融合タンパク質の蛍光タイムラプス イメージングを使用してを決定する方法について説明します。
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Alber, A. B., Suter, D. M. Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture. J. Vis. Exp. (132), e56604, doi:10.3791/56604 (2018).
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