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レプトスピラトランスポゾン挿入変異体中に黄金シリアのハムスター感染の測定フィットネスにハイスループット並列配列
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
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レプトスピラトランスポゾン挿入変異体中に黄金シリアのハムスター感染の測定フィットネスにハイスループット並列配列

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8,757 Views

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11:50 min

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December 18, 2017

DOI:

10.3791/56442-v

DOI:
10.3791/56442-v

•

11:50 min
December 18, 2017

•

1 Views
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1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine,David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology,David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics,University of California Los Angeles

Summary

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ここで高スループット シーケンス ハムスターの挑戦後トランスポゾン レプトスピラ突然変異体組織の定量化とトランスポゾン変異を組み合わせた手法について述べる。このプロトコルは、生存と動物の普及のため画面変異体に使え、生体外で研究にも適用することができます。

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High-throughput SequencingTransposon Insertion MutantsLeptospira InterrogansGolden Syrian Hamster InfectionIn Vivo FitnessIn Vitro FitnessVirulence GenesColonizationDisseminationSurvivalFiltration UnitEMJH PlatesKanamycinNested PCRTransposon Insertion Site

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Lourdault, K., Matsunaga, J., Evangelista, K. V., Haake, D. A. High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection. J. Vis. Exp. (130), e56442, doi:10.3791/56442 (2017).

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