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التسلسل الفائق موازية "قياس اللياقة البدنية" الإصابة إينتيروجانس Leptospira ينقول الإدراج طفرات خلال الذهبي السوري الهامستر
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
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التسلسل الفائق موازية "قياس اللياقة البدنية" الإصابة إينتيروجانس Leptospira ينقول الإدراج طفرات خلال الذهبي السوري الهامستر

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8,756 Views

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11:50 min

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December 18, 2017

DOI:

10.3791/56442-v

DOI:
10.3791/56442-v

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11:50 min
December 18, 2017

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1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine,David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology,David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics,University of California Los Angeles

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High-throughput SequencingTransposon Insertion MutantsLeptospira InterrogansGolden Syrian Hamster InfectionIn Vivo FitnessIn Vitro FitnessVirulence GenesColonizationDisseminationSurvivalFiltration UnitEMJH PlatesKanamycinNested PCRTransposon Insertion Site

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Lourdault, K., Matsunaga, J., Evangelista, K. V., Haake, D. A. High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection. J. Vis. Exp. (130), e56442, doi:10.3791/56442 (2017).

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