/
/
Personalizada péptido matrices para la detección de aloanticuerpos HLA en trasplante de órganos
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Division of Nephrology and Hypertension, and the Center for Kidney Research and Therapeutics at the Feinberg Cardiovascular Research Institute,Northwestern University Feinberg School of Medicine, 2Surgery-Organ Transplantation,Northwestern University Feinberg School of Medicine, 3School of Biological Sciences and Medical Engineering,Southeast University
Chapters
- 00:05Title
- 00:55Donor and Recipient HLA Sequence Bioinformatic Analysis and Custom Array Synthesis
- 03:19Probe the Pre- and Post-Transplant Sera
- 05:06Data Acquisition and Analysis
- 06:11Results: Representative Recipient anti-Donor HLA Alloantibody Detection
- 07:36Conclusion
Desajustes en las secuencias de leucocito humano antígeno (HLA) entre pares de receptores y donante de órganos son la causa principal de rechazo mediada por anticuerpos en el trasplante de órganos. Aquí presentamos el uso de matrices de antígeno personalizados basados en secuencias HLA de donantes individuales para sondear aloanticuerpos HLA de anti-donantes en receptores de órganos.
