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激光微辐照在哺乳动物细胞单、双链断裂修复中的应用
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
DOI:

08:18 min

September 05, 2017

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Chapters

  • 00:05Title
  • 00:31Laser Micro-irradiation
  • 04:02Live Cell Image Analysis of the Recruitment of Fluorescent Proteins to Micro-irradiated Sites
  • 05:54Results: Laser Micro-irradaition is a Tool for Characterizing DNA Damage and Repair
  • 07:13Conclusion

Summary

Automatic Translation

共焦荧光显微镜和激光微辐照提供的工具, 诱导 dna 损伤和监测的反应, dna 修复蛋白在选定的 sub-nuclear 地区。这项技术大大提高了我们对损伤检测、信号和招聘的认识。这份手稿演示了这些技术, 检查单和双链断裂修复。

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