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Multi-Photonen-Time Lapse Imaging Entwicklung in Echtzeit zu visualisieren: Visualisierung der Migration Neuralleisten-Zellen in den Zebrafish Embryos
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Developmental Biology
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JoVE Journal Developmental Biology
Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
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Multi-Photonen-Time Lapse Imaging Entwicklung in Echtzeit zu visualisieren: Visualisierung der Migration Neuralleisten-Zellen in den Zebrafish Embryos

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7,358 Views

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10:13 min

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August 09, 2017

DOI:

10.3791/56214-v

DOI:
10.3791/56214-v

•

10:13 min
August 09, 2017

•

8 Views
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1Department of Ophthalmology and Visual Sciences, Kellogg Eye Center,University of Michigan

Summary

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Eine Kombination der erweiterten optischen Techniken der Laser-scanning-Mikroskopie mit langen Wellenlänge Multi-Photonen Fluoreszenzanregung wurde implementiert, um hochauflösende, dreidimensionale, Echtzeit-Bildgebung der Neuralleiste Migration mit Tg (Sox10:EGFP) und Tg (Foxd3:GFP) Zebrafish Embryos zu erfassen.

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Cite this Article

Williams, A. L., Bohnsack, B. L. Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos. J. Vis. Exp. (126), e56214, doi:10.3791/56214 (2017).

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