Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Antionette L. Williams; Brenda L. Bohnsack

doi:10.3791/56214

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Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

JoVE Journal
発生生物学

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JoVE Journal 発生生物学

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

7,358 Views

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10:13 min

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August 09, 2017

DOI:

10.3791/56214-v

DOI:

10.3791/56214-v

•

10:13 min

August 09, 2017

•

8 Views

Antionette L. Williams¹, Brenda L. Bohnsack¹

¹Department of Ophthalmology and Visual Sciences, Kellogg Eye Center,University of Michigan

概要

A combination of the advanced optical techniques of laser scanning microscopy with long wavelength multi-photon fluorescence excitation was implemented to capture high-resolution, three-dimensional, real-time imaging of neural crest migration in Tg(sox10:EGFP) and Tg(foxd3:GFP) zebrafish embryos.

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Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

概要

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