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Cultivo In Vivo-como astrocitos murinos mediante el protocolo AWESAM rápido, sencillo y barato
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Neuroscience
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JoVE Journal Neuroscience
Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol
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Cultivo In Vivo-como astrocitos murinos mediante el protocolo AWESAM rápido, sencillo y barato

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8,218 Views

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07:56 min

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January 10, 2018

DOI:

10.3791/56092-v

DOI:
10.3791/56092-v

•

07:56 min
January 10, 2018

•

18 Views
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1Chemical Biology, Chemistry Research Laboratory,University of Oxford, 2Department of Physiology, Anatomy, and Genetics,University of Oxford, 3Trans-synaptic signaling,European Neuroscience Institute

Summary

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El protocolo AWESAM descrito aquí es óptimo para el cultivo de astrocitos murinos en aislamiento de otras células del cerebro de una manera rápida, sencilla y de bajo costo. Los astrocitos AWESAM exhibición espontáneo Ca2 + señalización, morfología y gene expresión perfiles similares a los astrocitos en vivo.

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Cite this Article

Wolfes, A. C., Dean, C. Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol. J. Vis. Exp. (131), e56092, doi:10.3791/56092 (2018).

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