Culturing <em>In Vivo</em>-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

Anne C. Wolfes; Camin Dean

doi:10.3791/56092

Journal

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Neuroscience

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Culture In Vivo-comme les Astrocytes Murine en utilisant le protocole AWESAM rapide, Simple et peu coûteux

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Neuroscience

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JoVE Journal Neuroscience

Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

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Culture In Vivo-comme les Astrocytes Murine en utilisant le protocole AWESAM rapide, Simple et peu coûteux

8,218 Views

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07:56 min

•

January 10, 2018

DOI:

10.3791/56092-v

DOI:

10.3791/56092-v

•

07:56 min

January 10, 2018

•

18 Views

Anne C. Wolfes^1,2,3, Camin Dean³

¹Chemical Biology, Chemistry Research Laboratory,University of Oxford, ²Department of Physiology, Anatomy, and Genetics,University of Oxford, ³Trans-synaptic signaling,European Neuroscience Institute

Summary

Le protocole AWESAM décrit ici est optimal pour la culture des astrocytes murines isolément des autres cellules du cerveau une manière rapide, simple et peu coûteux. AWESAM astrocytes présentent spontanée Ca^{2 +} signalisation, la morphologie et génétique des profils d’expression semblables aux astrocytes in vivo.

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Culture In Vivo-comme les Astrocytes Murine en utilisant le protocole AWESAM rapide, Simple et peu coûteux

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Summary

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