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Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol
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JoVE Journal Neurowissenschaften
Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

8,218 Views

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07:56 min

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January 10, 2018

DOI:

10.3791/56092-v

DOI:
10.3791/56092-v

•

07:56 min
January 10, 2018

•

18 Views
,
1Chemical Biology, Chemistry Research Laboratory,University of Oxford, 2Department of Physiology, Anatomy, and Genetics,University of Oxford, 3Trans-synaptic signaling,European Neuroscience Institute

Summary

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The AWESAM protocol described here is optimal for culturing murine astrocytes in isolation from other brain cells in a fast, simple, and inexpensive manner. AWESAM astrocytes exhibit spontaneous Ca2+ signaling, morphology, and gene expression profiles similar to astrocytes in vivo.

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Wolfes, A. C., Dean, C. Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol. J. Vis. Exp. (131), e56092, doi:10.3791/56092 (2018).

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