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3维球形共培养胰腺癌细胞和成纤维细胞的制备及代谢试验
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
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3维球形共培养胰腺癌细胞和成纤维细胞的制备及代谢试验

DOI:
10.3791/56081-v

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10:51 min

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August 23, 2017

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, , , , ,
1Molecular Medicine Division,Translational Genomics Research Institute, 2Agilent Technologies

Chapters

  • 00:05Title
  • 00:51Pancreatic Cancer 3D Spheroid Magnetic Bioprinting
  • 03:473D Pancratic Tumor Spheroids Extracellular Flux Analysis
  • 07:34Results: Representative Spheroid Growth and Functionality Analyses
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了一种制备3维 (3D) 椭球共培养胰腺癌细胞和成纤维细胞的方法, 其次是利用细胞外流量分析仪测量代谢功能。

Tags

3D SpheroidCo-culturePancreatic Cancer CellsFibroblastsMetabolic AssayExtracellular Flux AnalysisTumor MicroenvironmentCell MagnetizationNanoshuttleMagnetic Spheroid Drive

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