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Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

English

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15,109 Views

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08:15 min

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May 07, 2019

DOI:

10.3791/59567-v

DOI:
10.3791/59567-v

•

08:15 min
May 07, 2019

•

9 Views
, , , , , ,
1Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine,University of Helsinki, 2Cancer and Translational Medicine Research Unit,University of Oulu, 3Medical Research Center,Oulu University Horspital, 4Helsinki University Hospital

Summary

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Tumor microenvironment is an essential part of cancer growth and invasion. To mimic carcinoma progression, a biologically relevant human matrix is needed. This protocol introduces an improvement for the in vitro three-dimensional spheroid invasion assay by applying a human leiomyoma-based matrix. The protocol also introduces a computer-based cell invasion analysis.

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Cite this Article

Naakka, E., Tuomainen, K., Wistrand, H., Palkama, M., Suleymanova, I., Al-Samadi, A., Salo, T. Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay. J. Vis. Exp. (147), e59567, doi:10.3791/59567 (2019).

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