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Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
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Krebsforschung
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JoVE Journal Krebsforschung
Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

11,711 Views

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10:51 min

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August 23, 2017

DOI:

10.3791/56081-v

DOI:
10.3791/56081-v

•

10:51 min
August 23, 2017

•

5 Views
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1Molecular Medicine Division,Translational Genomics Research Institute, 2Agilent Technologies

Tags

3D SpheroidCo-culturePancreatic Cancer CellsFibroblastsMetabolic AssayExtracellular Flux AnalysisTumor MicroenvironmentCell MagnetizationNanoshuttleMagnetic Spheroid Drive

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Noel, P., Muñoz, R., Rogers, G. W., Neilson, A., Von Hoff, D. D., Han, H. Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts. J. Vis. Exp. (126), e56081, doi:10.3791/56081 (2017).

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