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Grânulo baseado Multiplex ensaio para análise de perfis de citocinas lágrima
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Immunology and Infection
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JoVE Journal Immunology and Infection
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Grânulo baseado Multiplex ensaio para análise de perfis de citocinas lágrima

português

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11,641 Views

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09:22 min

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October 13, 2017

DOI:

10.3791/55993-v

DOI:
10.3791/55993-v

•

09:22 min
October 13, 2017

•

10 Views
, , , , , ,
1National Healthcare Group Eye Institute,Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), 3Institute of Molecular and Cell Biology,A*STAR, 4GROW Research Laboratory,Narayana Nethralaya Foundation, 5Vimta Labs Limited

Summary

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Análise do filme lacrimal citocinas ajuda no estudo de várias doenças oculares. Grânulo baseado multiplex ensaios são simples e sensível e permitem o teste de alvos múltiplos em amostras com volumes pequenos. Aqui descrevemos um protocolo para lágrima filme cytokine perfis usando o um grânulo baseado multiplex do ensaio.

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Balne, P. K., AU, V. B., Tong, L., Ghosh, A., Agrawal, M., Connolly, J., Agrawal, R. Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles. J. Vis. Exp. (128), e55993, doi:10.3791/55993 (2017).

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