Journal
/
Immunology and Infection
/
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

English

Automatically Generated

11,641 Views

•

09:22 min

•

October 13, 2017

DOI:

10.3791/55993-v

DOI:
10.3791/55993-v

•

09:22 min
October 13, 2017

•

10 Views
, , , , , ,
1National Healthcare Group Eye Institute,Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), 3Institute of Molecular and Cell Biology,A*STAR, 4GROW Research Laboratory,Narayana Nethralaya Foundation, 5Vimta Labs Limited

Summary

Automatically generated

Analysis of tear film cytokines helps in studying various ocular diseases. Bead based multiplex assays are simple and sensitive and enable the testing of multiple targets in samples with small volumes. Here we describe a protocol for tear film cytokine profiling a using bead based multiplex assay.

Explore More Videos

Tear Cytokine ProfileBead-based Multiplex AssaySchirmer StripOphthalmologyCytokinesOcular DiseasesAnalytesTear SampleAssay BufferCentrifugationCytokine ProfilingMultiplex AssayCytokine StandardsAssay WorksheetMagnetic Plate Washer

Read Article

Cite this Article

Balne, P. K., AU, V. B., Tong, L., Ghosh, A., Agrawal, M., Connolly, J., Agrawal, R. Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles. J. Vis. Exp. (128), e55993, doi:10.3791/55993 (2017).

Download .ris file

Copy

Share Video

.

Copy