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Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

11,641 Views

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09:22 min

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October 13, 2017

DOI:

10.3791/55993-v

DOI:
10.3791/55993-v

•

09:22 min
October 13, 2017

•

10 Views
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1National Healthcare Group Eye Institute,Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), 3Institute of Molecular and Cell Biology,A*STAR, 4GROW Research Laboratory,Narayana Nethralaya Foundation, 5Vimta Labs Limited

Summary

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Analysis of tear film cytokines helps in studying various ocular diseases. Bead based multiplex assays are simple and sensitive and enable the testing of multiple targets in samples with small volumes. Here we describe a protocol for tear film cytokine profiling a using bead based multiplex assay.

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Balne, P. K., AU, V. B., Tong, L., Ghosh, A., Agrawal, M., Connolly, J., Agrawal, R. Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles. J. Vis. Exp. (128), e55993, doi:10.3791/55993 (2017).

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