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Assay voor fosforylatie en microtubulus bindende samen met lokalisatie van Tau-eiwit in Colorectal kankercellen
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Biochemistry
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JoVE Journal Biochemistry
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
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Assay voor fosforylatie en microtubulus bindende samen met lokalisatie van Tau-eiwit in Colorectal kankercellen

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8,832 Views

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12:55 min

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October 10, 2017

DOI:

10.3791/55932-v

DOI:
10.3791/55932-v

•

12:55 min
October 10, 2017

•

2 Views
, ,
1Systems Biotechnology Research Center,KIST Gangneung Institute of Natural Products, 2Division of Bio-Medical Science & Technology, KIST School,Korea University of Science and Technology

Summary

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Dit manuscript wordt beschreven standaardprotocollen voor het meten van tau hyperphosphorylation, tau binding aan de microtubuli, en lokalisatie van intracellulaire tau drug behandelingen na te meten. Deze protocollen kunnen herhaaldelijk worden gebruikt voor het screenen van drugs of andere verbindingen die gericht zijn op tau hyperphosphorylation of microtubulus bindend.

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Huda, M. N., Erdene-Ochir, E., Pan, C. Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells. J. Vis. Exp. (128), e55932, doi:10.3791/55932 (2017).

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