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实时 cAMP 检测气味受体激活的活细胞测量
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
DOI:

09:11 min

October 02, 2017

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Chapters

  • 00:05Title
  • 00:50Plating and Transfection of Hana3A Cells
  • 03:03Transfection of Plasmids
  • 05:24Stimulation and Measuring OR Activity Using the Real-time cAMP Assay
  • 07:27Results: Kinetic Measurements of the Response of OR5AN1 to Muscone
  • 08:23Conclusion

Summary

Automatic Translation

表征气味受体的功能在 deorphanization 过程中起着不可缺少的作用。我们描述了一种方法来测量气味受体的激活, 在实时使用阵营分析。

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