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实时 cAMP 检测气味受体激活的活细胞测量
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
Please note that all translations are automatically generated.
Click here for the English version.
实时 cAMP 检测气味受体激活的活细胞测量
DOI:
10.3791/55831-v
•
09:11 min
•
October 02, 2017
•
Yuetian Zhang*
1
,
Yi Pan*
1
,
Hiroaki Matsunami
3
,
Hanyi Zhuang
4
1
Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education
,
Shanghai Jiao Tong University School of Medicine
,
2
Department of Molecular Genetics and Microbiology
,
Duke University Medical Center
,
3
Department of Neurobiology, Duke Institute for Brain Sciences
,
Duke University Medical Center
,
4
Institute of Health Science
,
Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine
Chapters
00:05
Title
00:50
Plating and Transfection of Hana3A Cells
03:03
Transfection of Plasmids
05:24
Stimulation and Measuring OR Activity Using the Real-time cAMP Assay
07:27
Results: Kinetic Measurements of the Response of OR5AN1 to Muscone
08:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
表征气味受体的功能在 deorphanization 过程中起着不可缺少的作用。我们描述了一种方法来测量气味受体的激活, 在实时使用阵营分析。
Tags
Odorant Receptor
CAMP Assay
Live-cell Measurement
High-throughput Screening
Heterologous Expression System
Hana3A Cells
Transfection
Odorant-to-receptor Activation
Phase-contrast Microscopy
Cell Culture
Trypsinization
Cell Counting
96-well Plate
Incubation
Plasmid Constructs
Transfection Mixture
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