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En ny mammedisk fedtpudstransplantationsteknik til visualisering af fartøjsgenerering af vaskulære endotelceller
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
DOI:

12:41 min

August 03, 2017

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Chapters

  • 00:05Title
  • 01:26Isolation of VESCs from the Mouse Mammary Gland
  • 04:42In Vivo VESC Vessel Formation Assay Using the Mammay Fat Pad
  • 08:05Whole Mount Tissue Preparation for Microscopic Observation
  • 10:23Results: Isolation of Vascular Endothelial Stem Cells and Vessel Generation of Tranplanted Cells
  • 11:27Conclusion

Summary

Automatic Translation

Dette arbejde demonstrerer en ny tilgang til vurdering af proliferation, differentiering og fartøjsdannende potentiale af vaskulære endotelceller (VESC'er) gennem brystfedtpudstransplantation efterfulgt af helbårne vævsblandinger til mikroskopisk observation. En linjesporingstrategi til undersøgelse af VESCs adfærd in vivo præsenteres også.

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