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A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
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A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

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12:41 min

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August 03, 2017

DOI:

10.3791/55795-v

DOI:
10.3791/55795-v

•

12:41 min
August 03, 2017

•

3 Views
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1State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences

Summary

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This work demonstrates a novel approach to assess the proliferation, differentiation, and vessel-forming potential of vascular endothelial stem cells (VESCs) through mammary fat pad transplantation followed by whole-mount tissue preparation for microscopic observation. A lineage tracing strategy to investigate the behavior of VESCs in vivo is also presented.

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Mammary Fat PadVascular Endothelial Stem CellsVessel GenerationTransplantation TechniqueAngiogenic EnvironmentCell IsolationMicroscopic Observation

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Cite this Article

Yu, Q. C., Song, W., Lai, D., Zeng, Y. A. A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells. J. Vis. Exp. (126), e55795, doi:10.3791/55795 (2017).

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