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利用 Immunolabeling 分析斑马鱼胚胎中的稳定、动态和初生的微管
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
DOI:

12:38 min

September 20, 2017

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Chapters

  • 00:05Title
  • 00:45Fixation of Staged Embryos
  • 01:52Sectioning for Immunolabeling
  • 04:04Confocal Imaging
  • 05:17Quantifying Microtubules
  • 07:10De Novo MT Assembly Assay
  • 09:05Depolymerize Existing MTs
  • 10:26Results: Analysis of Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo
  • 12:00Conclusion

Summary

Automatic Translation

Immunolabeling 的方法来分析不同群体的微管在发展斑马鱼大脑中描述, 这是广泛适用于其他组织。第一个协议概述了 immunolabeling 稳定和动态微管的优化方法。第二个协议提供了一种具体的图像和量化初生微管的方法。

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